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Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from <t>16S</t> <t>rRNA</t> gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.
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Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from <t>16S</t> <t>rRNA</t> gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.
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Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from 16S rRNA gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Longitudinal remodeling of gastric microbiota following Helicobacter pylori eradication reveals an eradication-associated microbial signature in gastric cancer

doi: 10.3389/fcimb.2026.1848437

Figure Lengend Snippet: Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from 16S rRNA gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: The raw 16S rRNA gene sequencing data generated in this study have been deposited in the National Center for Biotechnology Information Sequence Read Archive under the BioProject accession number PRJNA1447187 and are publicly available at http://www.ncbi.nlm.nih.gov/bioproject/1447187 .

Techniques: Functional Assay, Infection, Comparison, Sequencing

Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from 16S rRNA gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.

Journal: bioRxiv

Article Title: Taxonomic Composition and Predicted Functional Potential of a Commercial Microbiome-Based Fertilizer Additive and Agricultural Soils in Eastern Paraguay

doi: 10.64898/2026.06.03.729874

Figure Lengend Snippet: Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from 16S rRNA gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.

Article Snippet: 16S rRNA gene sequencing was performed by Azenta Life Sciences (South Plainfield, NJ, USA) using their 16S-EZ service.

Techniques: Sequencing, Functional Assay